Document 0543
 DOCN  M9460543
 TI    Identification of an HIV-1 Nef peptide that binds to HLA class II
       antigens.
 DT    9408
 AU    Torres BA; Johnson HM; Department of Microbiology and Cell Science,
       University of; Florida, Gainesville 32611.
 SO    Biochem Biophys Res Commun. 1994 Apr 29;200(2):1059-65. Unique
       Identifier : AIDSLINE MED/94234994
 AB    Overlapping peptides corresponding to the entire Nef (HIVBRU) sequence
       were tested for their relative abilities to block binding of
       staphylococcal enterotoxins (SEs) to Raji cells. An internal sequence,
       Nef(123-160), blocked binding of two highly homologous SEs, SEA and SEE,
       while it was less effective against SEB and SEC1. Nef(123-160) bound
       directly class II DR antigens, as assessed by specific antibodies, and
       its binding was significantly inhibited by SEE, and also by SEA to a
       lesser degree. Purified Nef inhibited specific binding of SEA to Raji
       cells in a dose-dependent manner. Nef induced IL 2 production and
       proliferation by human mononuclear cells. Definitive expansion of
       specific V beta T cell populations was not observed, possibly due to
       lesser mitogenic activity of Nef relative to SEs. Thus, Nef may have
       mitogenic activity similar to that of superantigens.
 DE    Amino Acid Sequence  Binding Sites  Binding, Competitive  Cell Division
       Cell Line  Enterotoxins/METABOLISM  Gene Products,
       nef/GENETICS/*METABOLISM  Human  HIV-1/GENETICS/IMMUNOLOGY/*METABOLISM
       HLA-D Antigens/*METABOLISM  Interleukin-2/BIOSYNTHESIS  Leukocytes,
       Mononuclear/CYTOLOGY/IMMUNOLOGY/METABOLISM  Molecular Sequence Data
       Peptide Fragments/GENETICS/METABOLISM  Superantigens  Support, U.S.
       Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).